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oligo sequences for guide rna targeting lamp2a, prdx1 and crtc1  (ATUM Bio)
90
ATUM Bio oligo sequences for guide rna targeting lamp2a, prdx1 and crtc1
LAMP2a targets PRDX1 and <t>CRTC1</t> to modulate macrophages activation. (a) LAMP2a expression in TS-stimulated BMDMs which were treated by bafilomycin (Bafilo) or not. (b) Protein level of GPADH binding to LAMP2a in TS-stimulated BMDMs treated by bafilomycin (Bafilo) or not. (c) LAMP2a, PRDX1, CRTC1 and IRG1 expression in co-IP experiments of TS-stimulated BMDMs treated by bafilomycin (Bafilo) or not. All the IP-proteins were immunoprecipitated by anti-LAMP2a antibody and detected by respective antibodies. (d) Protein level of PRDX1, CRTC1 and IRG1 in TS-stimulated BMDMs which were transfected by sh-NC, sh-L2a or not. (e) The surface plasmon resonances (SPR) of LAMP2a-bound PRDX1, CRTC1 and IRG proteins. The results were fitted as dissociation constants (KDs). (f) Protein level of LAMP2a, PRDX1 and CRTC1 in TS-stimulated, genetically modified mouse HSCs-derived macrophages. “SCR” represents sg-SRC vector and “V” represents overexpression control vector. (g) Heatmap of relative mRNA expression of macrophage activation-related genes in mouse HSCs-derived macrophages described as ( f ). “pMIG” stands for overexpression control vector. Data were measured by qPCR, normalized to corresponding groups as noted, represented as log2 scale, with β-actin as control. (h) LDH release of 4 T1, CT26, LL/2 cells after 24 h co-culture with genetically modified HSCs-derived macrophages at a ratio of 40:1 as E:T. The percentage of cytotoxicity was calculated by maximum tumor cells LDH release control. (i) CREB and CEBP/β expression in TS-stimulated BMDMs treated by sh-NC, sh-L2a or not. Total CREB protein was shown as pan-CREB, and S133-phosphorylated CREB was shown as p-CREB. (j) Luminescence assays of H 2 O 2 production in BMDMs which were transfected by shRNA after TS or normal medium culture. (k) Illustration of mechanism for LAMP2a-PRDX1/CRTC1 axis.
Oligo Sequences For Guide Rna Targeting Lamp2a, Prdx1 And Crtc1, supplied by ATUM Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
oligo sequences for guide rna targeting lamp2a, prdx1 and crtc1 - by Bioz Stars, 2026-08
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LAMP2a targets PRDX1 and CRTC1 to modulate macrophages activation. (a) LAMP2a expression in TS-stimulated BMDMs which were treated by bafilomycin (Bafilo) or not. (b) Protein level of GPADH binding to LAMP2a in TS-stimulated BMDMs treated by bafilomycin (Bafilo) or not. (c) LAMP2a, PRDX1, CRTC1 and IRG1 expression in co-IP experiments of TS-stimulated BMDMs treated by bafilomycin (Bafilo) or not. All the IP-proteins were immunoprecipitated by anti-LAMP2a antibody and detected by respective antibodies. (d) Protein level of PRDX1, CRTC1 and IRG1 in TS-stimulated BMDMs which were transfected by sh-NC, sh-L2a or not. (e) The surface plasmon resonances (SPR) of LAMP2a-bound PRDX1, CRTC1 and IRG proteins. The results were fitted as dissociation constants (KDs). (f) Protein level of LAMP2a, PRDX1 and CRTC1 in TS-stimulated, genetically modified mouse HSCs-derived macrophages. “SCR” represents sg-SRC vector and “V” represents overexpression control vector. (g) Heatmap of relative mRNA expression of macrophage activation-related genes in mouse HSCs-derived macrophages described as ( f ). “pMIG” stands for overexpression control vector. Data were measured by qPCR, normalized to corresponding groups as noted, represented as log2 scale, with β-actin as control. (h) LDH release of 4 T1, CT26, LL/2 cells after 24 h co-culture with genetically modified HSCs-derived macrophages at a ratio of 40:1 as E:T. The percentage of cytotoxicity was calculated by maximum tumor cells LDH release control. (i) CREB and CEBP/β expression in TS-stimulated BMDMs treated by sh-NC, sh-L2a or not. Total CREB protein was shown as pan-CREB, and S133-phosphorylated CREB was shown as p-CREB. (j) Luminescence assays of H 2 O 2 production in BMDMs which were transfected by shRNA after TS or normal medium culture. (k) Illustration of mechanism for LAMP2a-PRDX1/CRTC1 axis.

Journal: EBioMedicine

Article Title: Tumor cells induce LAMP2a expression in tumor-associated macrophage for cancer progression

doi: 10.1016/j.ebiom.2019.01.045

Figure Lengend Snippet: LAMP2a targets PRDX1 and CRTC1 to modulate macrophages activation. (a) LAMP2a expression in TS-stimulated BMDMs which were treated by bafilomycin (Bafilo) or not. (b) Protein level of GPADH binding to LAMP2a in TS-stimulated BMDMs treated by bafilomycin (Bafilo) or not. (c) LAMP2a, PRDX1, CRTC1 and IRG1 expression in co-IP experiments of TS-stimulated BMDMs treated by bafilomycin (Bafilo) or not. All the IP-proteins were immunoprecipitated by anti-LAMP2a antibody and detected by respective antibodies. (d) Protein level of PRDX1, CRTC1 and IRG1 in TS-stimulated BMDMs which were transfected by sh-NC, sh-L2a or not. (e) The surface plasmon resonances (SPR) of LAMP2a-bound PRDX1, CRTC1 and IRG proteins. The results were fitted as dissociation constants (KDs). (f) Protein level of LAMP2a, PRDX1 and CRTC1 in TS-stimulated, genetically modified mouse HSCs-derived macrophages. “SCR” represents sg-SRC vector and “V” represents overexpression control vector. (g) Heatmap of relative mRNA expression of macrophage activation-related genes in mouse HSCs-derived macrophages described as ( f ). “pMIG” stands for overexpression control vector. Data were measured by qPCR, normalized to corresponding groups as noted, represented as log2 scale, with β-actin as control. (h) LDH release of 4 T1, CT26, LL/2 cells after 24 h co-culture with genetically modified HSCs-derived macrophages at a ratio of 40:1 as E:T. The percentage of cytotoxicity was calculated by maximum tumor cells LDH release control. (i) CREB and CEBP/β expression in TS-stimulated BMDMs treated by sh-NC, sh-L2a or not. Total CREB protein was shown as pan-CREB, and S133-phosphorylated CREB was shown as p-CREB. (j) Luminescence assays of H 2 O 2 production in BMDMs which were transfected by shRNA after TS or normal medium culture. (k) Illustration of mechanism for LAMP2a-PRDX1/CRTC1 axis.

Article Snippet: The oligo sequences for guide RNA targeting Lamp2a , Prdx1 and Crtc1 were designed by DNA 2·0, with three to five candidates of highest scores obtained.

Techniques: Activation Assay, Expressing, Binding Assay, Co-Immunoprecipitation Assay, Immunoprecipitation, Transfection, Genetically Modified, Derivative Assay, Plasmid Preparation, Over Expression, Control, Co-Culture Assay, shRNA